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KMID : 1240020180220000139
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International Neurourology Journal
2018 Volume.22 No. 0 p.139 ~ p.146
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Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice
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Moon Eun-Jin
Ko Il-Gyu
Kim Sung-Eun
Jin Jun-Jang
Hwang Lak-Kyong
Kim Chang-Ju
An Hyeon-Jun
Lee Bong-Jae
Yi Jae-Woo
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Abstract
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Purpose: Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice.
Methods: For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed.
Results: Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment.
Conclusions: In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.
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KEYWORD
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Sleep deprivation, Dexmedetomidine, Depression, Serotonin, Dopamine
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